Phospho-SMAD3 (Ser425) Monoclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-51043-60, E-AB-51043-120, E-AB-51043-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Phospho-SMAD3 (Ser425)
Target Synonym: DKFZP586N0721; DKFZp686J10186; hMAD 3; hMAD-3; hSMAD3; HSPC193; HST17436; JV15 2; JV15-2; JV152; LDS1C; LDS3; MAD (mothers against decapentaplegic Drosophila) homolog 3; MAD homolog 3; Mad homolog JV15 2; Mad protein homolog; MAD; mothers against decapentaplegic homolog 3; Mad3; MADH 3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD family member 3; SMAD; mothers against DPP homolog 3; Smad3; SMAD3
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Application: IHC-p
Isotype: IgG
Clonality: Monoclonal
Clone No.: Clone:4C5
UNIProt ID: P84022
Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Concentration: 1 mg/mL
Immunogen: Synthetic Peptide of Phospho-Smad3 (Ser425)
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method: Protein A purification
Dilution: IHC 1:50-300
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only