PML/RARA Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-92777-60, E-AB-92777-120, E-AB-92777-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PML/RARA
Target Synonym: PML/RARA
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P29590/P10276
Background: Promyelocytic leukemia/retinoic acid receptor alpha or PML-RARA refers to an abnormal fusion gene sequence. It is a specific rearrangement of genetic material from two separate chromosomes (chromosomal translocation) and is associated with a specific type of leukemia.Promyelocytic leukemia (PML) is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Retinoic acid receptor alpha(RARA), regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human PML/RARA
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 47-48kDa/62-97kDa/39kDa/50kDa
Observed MW: 51kDa/130kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only