QuicKey Pro Mouse IgG2c (Immunoglobulin G2c) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-M0010-24, E-OSEL-M0010-48, E-OSEL-M0010-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: IgG2c
UNIProt ID: IgG2c
Target Synonyms: IgG2c
Research Areas: Immunology
Target Species: Mouse
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgG2c concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 0.19 ng/mL
Detection Range: 0.31-20 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2c. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG2c are added to the micro ELISA plate wells. Mouse IgG2c in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG2c.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG2c. The concentration of Mouse IgG2c in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Mouse IgG2c in samples. No significant cross-reactivity or interference between Mouse IgG2c and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag