Rat ADP/Acrp30 (Adiponectin) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-R0006-24, E-OSEL-R0006-48, E-OSEL-R0006-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: ADP; Acrp30
UNIProt ID: A0A0G2K845
Target Synonyms: Adiponectin; GBP28; ACDC; APM1; ADPN; AdipoQ; ADIPQTL1;
Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells
Target Species: Rat
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 6.43 ng/mL
Detection Range: 31.25-1000 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Rat ADP/Acrp30 in samples. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag