Recombinant 2019-nCoV NSP10 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSR030471-10, PKSR030471-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NSP10
Target Synonym: SARS-CoV 2 nsp10; SARS-CoV 2 Growth factor-like peptide; SARS-CoV 2 GFL
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: N-6His
Accession: YP_009725306.1
Background: Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing.
Sequence: Ala1-Gln139
Purity: > 80 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.9 kDa
Observed MW: 18 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only