Recombinant 2019-nCoV Nucleocapsid Protein (His Tag)
Size: 100μg
Catalogue Number: PDEV100003-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nucleocapsid
Target Synonym: 2019-nCoV coronavirus NP Protein, 2019-nCoV np Protein, 2019-nCoV novel coronavirus Nucleoprotein Protein
UNIProt ID: P0DTC9
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: N-His
Accession: P0DTC9
Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Sequence: Met1-Ala419
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.4 kDa
Observed MW: 50-60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only