Recombinant 2019-nCoV Nucleoprotein / NP Protein (His Tag)
Size: 100μg
Catalogue Number: PKSR030506-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nucleoprotein, NP
Target Synonym: 2019-nCoV coronavirus NP Protein, 2019-nCoV np Protein, 2019-nCoV novel coronavirus Nucleoprotein Protein
Target Species: SARS-CoV-2
Expression Host: Baculovirus-Insect Cells
Fusion Tag: C-6His
Accession: NP_828858.1
Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Sequence: Met1-Ala422
Purity: > 80 % as determined by reducing SDS-PAGE.
Formulation: LyopHilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
Observed MW: 47.1 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only