Recombinant Caspase 3 Monoclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: E-AB-81444-50, E-AB-81444-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Caspase 3
Target Synonym: A830040C14Rik; Apopain; CASP-3; CASP3; CASP3; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama
Research Areas: Cancer, Cell Biology, Metabolism, Neuroscience
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB, IHC-P
Isotype: IgG
Clonality: Rabbit Monoclonal
Clone No.: R06-1B2
UNIProt ID: P42574
Background: Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Concentration: 300 μg/mL
Immunogen: A synthetic peptide of human Caspase 3
Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method: Affinity Purified
Dilution: WB 1:500-1:1000 IHC 1:50-1:100
Calculated MW: 32kDa
Observed MW: 32kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only