Recombinant Cynomolgus LAG-3/CD223 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSQ050011-10, PKSQ050011-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LAG-3; CD223
Target Symptom: Lymphocyte Activating 3; LAG3; LAG-3; CD223
Target Species: Cynomolgus macaques
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: N-His
Accession: XP_005570011.1
Background: Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3' s main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.
Activity: Immobilized Cynomolgus LAG-3-His at 2μg/ml(100 μl/well) can bind Human FGL1-Fc. The ED50 of Cynomolgus LAG-3-His is 5. 8 ug/ml.
Sequence: Pro23-Leu450(Pro74)
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, PH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.3 kDa
Observed MW: 55-65&140 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only