Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSQ050112-10, PKSQ050112-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Siglec-15; CD33L3
Target Symptom: Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3
Target Species: Cynomolgus macaques
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: A0A2K5UY47
Accession: A0A2K5UY47
Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Activity: Immobilized Cynomolgus Siglec-15-Fc(Cat#PKSQ050112) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 20.9 ng/ml.
Sequence: Phe20-Thr263
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 53.1 kDa
Observed MW: 55-70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only