Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSQ050059-10, PKSQ050059-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Uida; Beta-Gus
Target Synonym: Beta-glucuronidase; uidA; GUS
UNIProt ID: P05804
Target Species: E.coli
Expression Host: E.coli
Fusion Tag: N-His
Accession: P05804
Background: Beta-D-glucuronidase from E.coli is a -highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Sequence: Met1-Gln603
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 69.9 kDa
Observed MW: 72 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only