Recombinant HIV p24 protein (C-6His)
Size: 1mg
Catalogue Numbers: PKSV030277-1
Citations, Manuals and MSDS Available upon request.
Abbreviation: p24
Target Synonym: Capsid protein p24; Human immunodeficiency virus 1; Human immunodeficiency virus type 1 p24
UNIProt ID: P12493
Target Species: HIV
Expression Host: E.coli
Fusion Tag: C-6His
Accession: P12493
Background: The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
Sequence: Met142-Gly355
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μM filtered solution of PBS, pH7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: Please contact us for more information.
Calculated MW: 24.6 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only