Recombinant Human β-NGF/NGFB Protein (with pro peptide)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033552-10, PKSH033552-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: β-NGF; NGFB
Target Symptom: Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB
Research Area: Neuroscience
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P01138
Accession: P01138
Background: The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges; whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 32697 pg/mL.
Sequence: Glu19-Ala241
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 25 kDa
Observed MW: 30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only