Recombinant Human 4-1BB/TNFRSF9 Protein (Fc & His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032027-10, PKSH032027-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: 4-1BB; TNFRSF9
Target Symptom: CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA
Research Area: Cell biology; Cancer; immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc-6His
UNIProt ID: Q07011
Accession: Q07011
Background: Tumor necrosis factor receptor superfamily member 9(TNFRSF9) is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. It is absent from naive T cells; but upregulated and continually expressed following T cell activation. It is a receptor for TNFSF9/4-1BBL; and possibly active during T cell activation.
Activity: Immobilized Human 4-1BBL-His(Cat: PKSH032023) at 0.03μg/ml(100 μl/well) can bind Human 4-1BB-Fc. The ED50 of Human 4-1BB-Fc is 7.22 ug/ml.
Sequence: Leu24-Gln186
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44.0 kDa
Observed MW: 55-75 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only