Recombinant Human 4-1BBL/TNFSF9 Protein (His Tag)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH032023-20, PKSH032023-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: 4-1BBL; TNFSF9
Target Symptom: Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9
Research Area: Cancer; immunology
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: P41273
Accession: P41273
Background: Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain; placenta; lung; skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells; and is thought to be involved in T cell-tumor cell interaction.
Activity: Immobilized Human 4-1BB-His(Cat: PKSH032025) at 10μg/ml(100 μl/well) can bind Biotinylated Human 4-1BBL-His. The ED50 of Human 4-1BBL-His is 147.3 ug/ml.
Sequence: Arg71-Glu254
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 41.8 kDa
Observed MW: 16-20 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only