Recombinant Human ADA protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100156-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: ADA
Target Synonym: ADA; ADA1; adenine deaminase; Adenosine aminohydrolase; Adenosine Deaminase; EC 3.5.4.4
UNIProt ID: P00813
Research Areas: Cancer; Epigenetics and Nuclear Signaling; Metabolism
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P00813
Background: Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy-adenosine to inosine and deoxyinosine. ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis. Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co-activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion.
Sequence: Lys 11-Val 280
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 29.59 kDa
Observed MW: 32 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only