Recombinant Human AKR1C4 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032056-10, PKSH032056-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: AKR1C4
Target Synonym: Aldo-Keto Reductase Family 1 Member C4; 3-Alpha-HSD1; 3-Alpha-Hydroxysteroid Dehydrogenase Type I; Chlordecone Reductase; CDR; Dihydrodiol Dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4; CHDR
UNIProt ID: P17516
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P17516
Background: Aldo-Keto Reductase 1C4/AKR1C4 is a member of the aldo/keto reductase family that consists of more than 40 known enzymes and proteins. AKR1C4 has highly expressed in Liver. It can catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. AKR1C4 catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-α-Androstan-3-α, 17-β-diol (3-α-diol). In addition, AKR1C4 also has some 20-α-Hydroxysteroid Dehydrogenase activity.
Sequence: Met 1-Tyr323
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 39.3 kDa
Observed MW: 26&38 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only