Recombinant Human ALK-5/TGFBR1 (C-Fc)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033907-10, PKSH033907-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ALK-5; TGFBR1
Target Symptom: AAT5; activin A receptor type II-like kinase; 53kD; ACVRLK4; ALK-5; ALK-5ALK5; LDS1A; LDS2A; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1
Research Area: Signal Transduction; Cardiovascular; Cancer; metabolism; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-Fc
UNIProt ID: P36897
Accession: P36897
Background: TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII. Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells. Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development.
Sequence: Leu34-Glu125
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 37.2 kDa
Observed MW: 40-60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only