Recombinant Human Alkaline Phosphatase/ALPI Protein (Fc Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030691-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Alkaline Phosphatase; ALPI
Target Symptom: IAP
Research Area: Signal Transduction; Tags & Cell Markers; Cell biology; Cancer; immunology; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-hFc
UNIProt ID: P09923
Accession: P09923
Background: Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases; which in turn phosphorylate several proteins; including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due; at least in part; to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1; in which Tyr466 is changed to phenylalanine; can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
Activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 10, 000 pmoles/min/μg.
Sequence: Met 1-Asp 503
Purity: > 83 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 79.5 kDa
Observed MW: 90-95 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only