Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (Active)
Size: 50μg
Catalogue Number: PKSH030311-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: AMPK Heterotrimer
Target Symptom: AMPK; AMPKa1
Research Area: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His & GST Tag
UNIProt ID: P54619&O43741&P54646
Accession: P54619&O43741&P54646
Activity: The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Sequence: Met 1-Pro 331&Met 1-Ile 272&Met 1-Arg 552
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% glycerol, 1mM GSH, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 158 (38+30+90) kDa
Observed MW: 40; 42 & 95 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only