Recombinant Human AMPK1 protein (GST tag)
Size: 100μg
Catalogue Numbers: PDEH100136-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: AMPK1
Target Synonym: PRKAA1; AMPK1; AMPK subunit alpha-1; PRKAA1; ACACA kinase; PRKAA1
UNIProt ID: Q13131
Research Areas: Neuroscience; Signal Transduction; Cardiovascular; Cancer; Metabolism
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
Accession: Q13131-2
Background: AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
Sequence: Lys 421-Gln 574
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 41.83 kDa
Observed MW: 45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only