Recombinant Human APC protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100301-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: APC
Target Synonym: Adenomatous polyposis coli protein; APC;
Deleted in polyposis 2.5
UNIProt ID: P25054
Research Areas: cancer; Cardiovascular;
cell Biolog;
Epigenelics ond Nuclear signaling;
Neuros
science; stem cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P25054
Background: APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence), and PSD-93 domains. These domains bind microtubules, mitotic spindles, and F-actin and are involved in cell cycle, division, and migration. The immunogen does not include the beta -catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.
Sequence: Pro 2712-Val 2843
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 14.41 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only