Recombinant Human Arginase-1/ARG1 Protein (E.coli, His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032091-10, PKSH032091-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Arginase-1; ARG1
Target Symptom: Arginase-1; Liver-type arginase; Type I arginase; ARG1
Research Area: Signal Transduction; metabolism;
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: C-6His
UNIProt ID: P05089
Accession: P05089
Background: ARG1 is a member of the ureohydrolase family of enzymes. ARG1 can catalyze the hydrolysis of arginine to ornithine and urea. In the urea cycle, ARG1 catalyzes the fifth and final step, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. ARG1 is a cytosolic enzyme and expressed widely in the liver as part of the urea cycle. Inherited deficiency of this ARG1 causes argininemia, which is an autosomal recessive disorder characterized by hyperammonemia.
Activity: Measured by the production of urea during the hydrolysis of arginine. The specific activity is 6136. 25 pmol/min/µg.
Sequence: Met 1-lys322
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 20% Glycerol, 1mM DTT, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 35.8 kDa
Observed MW: 40 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only