Recombinant Human ASS1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032092-10, PKSH032092-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ASS1
Target Synonym: Argininosuccinate Synthase; Citrulline--Aspartate Ligase; ASS1; ASS
UNIProt ID: P00966
Research Areas: Signal Transduction; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P00966
Background: Argininosuccinate Synthase (ASS1) is an urea cycle enzyme with a tetrameric structure composed of identical subunits. ASS1 is involved in the synthesis of arginine and catalyzes that condensation of citrulline and aspartate to argininosuccinate using ATP. ASS1 is important to the urea cycle as it catalyzes the important second last step in the arginine biosynthetic pathway. ASS1 mainly expressed in periportal hepatocytes, but also in most other body tissues. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation.
Sequence: Met 1-Lys412
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 50mM Imidazole, 1mM DTT, 40% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 42.8 kDa
Observed MW: 50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only