Recombinant Human ATG4A Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032099-10, PKSH032099-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ATG4A
Target Synonym: Cysteine Protease ATG4A; AUT-Like 2 Cysteine Endopeptidase; Autophagin-2; Autophagy-Related Cysteine Endopeptidase 2; Autophagy-Related Protein 4 Homolog A; hAPG4A; ATG4A; APG4A; AUTL2
UNIProt ID: Q8WYN0
Research Areas: Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: Q8WYN0
Background: Cysteine Protease ATG4A (ATG4A) is a cytoplasmic protein that belongs to the peptidase C54 family. ATG4A is widely expressed in many tissues at a low level, but the highest expression is observed in skeletal muscle and brain. ATG4A is a cysteine protease required for autophagy; it cleaves the C-terminal part of MAP1LC3, GABARAPL2 or GABARAP. ATG4A is inhibited by N-ethylmaleimide. It is suggested that ATG4A has a significant role in suppressing various cancers.
Sequence: Met 1-Val398
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
Observed MW: 45-55 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only