Recombinant Human BDNF protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100216-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: BDNF
Target Synonym: Brain-Derived Neurotrophic Factor; BDNF; Abrineurin
UNIProt ID: P23560
Research Areas: Cardiovascular; Neuroscience; metabolism
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P23560
Background: The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
Sequence: His 129-Arg 247
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 12.98 kDa
Observed MW: 15 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only