Recombinant Human Beta-2-Microglobulin/B2M Protein (HEK293 Cells, His Tag)
Size: 100μg
Catalogue Number: PKSH030949-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Beta-2-Microglobulin, B2M
Target Synonym: Beta-2-Microglobulin, B2M, β2-Microglobulin
Research Areas: Cardiovascular, Cancer
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: NP_004039.1
Background: B2M, also known as &beta, 2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(&beta, 2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(&beta, 2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(&beta, 2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the&alpha, 3 chain. Unlike&alpha, 3, &beta, 2 has no transmembrane region. Directly above &beta, 2 lies the &alpha, 1 chain, which itself is lateral to the &alpha, 2. In the absence of B2M(&beta, 2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(&beta, 2 microglobulin) can indicate non-progression of HIV.
Sequence: Met 1-Met 119
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 13.5 kDa
Observed MW: 13.5 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only