Recombinant Human Biglycan/BGN protein (His tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100070-20, PDMH100070-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Biglycan; BGN
Target Synonym: Biglycan; Bone/Cartilage Proteoglycan I; PG-S1; BGN; SLRR1A
UNIProt ID: P21810
Research Areas: Signal Transduction; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P21810
Background: Biglycan is a 200-350 kD proteoglycan consisting of a 45 kD core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains. Biglycan binds to TGF--. It also binds to collagen type I in low ionic strength (less than 3 mM phosphate) buffer. At higher ionic strengths, Biglycan does not bind to collagen type I. It enhances the inhibition effect of TGF-- on osteoclast proliferation at a concentration of 4-20 mg/ml. It also prevents the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/ml.
Sequence: Glu20-Lys368
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 40.37 kDa
Observed MW: 42 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only