Recombinant Human BLNK/Ly-57 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033753-10, PKSH033753-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: BLNK; Ly-57
Target Symptom: B-Cell Linker Protein; B-Cell Adapter Containing a SH2 Domain Protein; B-Cell Adapter Containing a Src Homology 2 Domain Protein; Cytoplasmic Adapter Protein; Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa; SLP-65; BLNK; BASH; SLP65
Research Area: Signal Transduction; immunology;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-His
UNIProt ID: Q8WV28
Accession: Q8WV28
Background: B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.
Sequence: Met1-Ser456
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 51.5 kDa
Observed MW: 40-80 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only