Recombinant Human BPHL Protein (His Tag)
Size: 100μg
Catalogue Number: PKSH030575-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: BPHL
Target Synonym: BPH-RP, MCNAA, VACVASE
UNIProt ID: Q86WA6
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: Q86WA6-1
Background: BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.
Sequence: Ser38-Gln291
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 50mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 30.7 kDa
Observed MW: 31 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only