Recombinant Human Brain Natriuretic Peptide/BNP Protein (His & Flag Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032127-10, PKSH032127-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Brain Natriuretic Peptide; BNP
Target Synonym: Natriuretic peptides B; Gamma-brain natriuretic peptide; NPPB; BNP
UNIProt ID: P16860
Research Areas: Signal Transduction; Cardiovascular; Neuroscience;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His-Flag
Accession: P16860
Background: Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP); a useful marker of heart failure (HF); is considered to be secreted mainly from the ventricle; increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.
Sequence: His27-Arg102
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 11.0 kDa
Observed MW: 14 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only