Recombinant Human Calmegin/CLGN Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032147-10, PKSH032147-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Calmegin; CLGN
Target Synonym: Calmegin; CLGN
UNIProt ID: O14967
Research Areas: Signal Transduction; Developmental Biology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: O14967
Background: Calmegin (CLGN) is a member of the calreticulin family. Calmegin is a testis-specific endoplasmic reticulum chaperone protein. The functions during spermatogenesis as a chaperone for a range of client proteins that is important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. It is required for normal male fertility, normal sperm migration from the uterus into the oviduct. Calmegin binds calcium ions and interacts with PDILT. Calmegin may play a role in spermatogeneisis and infertility.
Sequence: Glu20-Trp471
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 52.9 kDa
Observed MW: 54 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only