Recombinant Human CALR/Calreticulin Protein (His Tag)
Size: 50μg
Catalogue Numbers: PKSH030606-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CALR; Calreticulin
Target Synonym: cC1qR; CRT; HEL-S-99n; RO; SSA
UNIProt ID: P27797
Research Areas: Signal Transduction; Tags & Cell Markers;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P27797
Background: Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction); rendering it inactive. The Ca2+ is bound with low affinity; but high capacity; and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum; calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo; as well as retinoic acid-induced neuronal differentiation. Therefore; calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
Sequence: Met 1-Ala413
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.4 kDa
Observed MW: 58 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only