Recombinant Human Carbonic Anhydrase 10/CA10 Protein (Human Cells, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032157-10, PKSH032157-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase 10; CA10
Target Synonym: Carbonic Anhydrase-Related Protein 10; Carbonic Anhydrase-Related Protein X; CA-RP X; CARP X; Cerebral Protein 15; CA10; CA-RPX; CARPX; HUCEP-15
UNIProt ID: Q9NS85
Research Areas: Neuroscience; epigenetics and nuclear signal
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q9NS85
Background: Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes; which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration; renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e.; the reversible hydration of CO2) due to point mutations in the zinc binding site; it has esterase activity. The human and mouse CA10 are expressed in the brain; indicating that they may play a role in brain development.
Sequence: Gln22-Asn300
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 32.8 kDa
Observed MW: 34 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only