Recombinant Human Carbonic Anhydrase 8/CA8 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031586-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase 8; CA8
Target Symptom: Carbonic Anhydrase-Related Protein; CARP; Carbonic Anhydrase VIII; CA-VIII; CA8; CALS; CAMRQ3; MGC120502; MGC99509
Research Area: Cancer
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: C-His
Accession: NP_004047.3
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Activity: Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg.
Sequence: Met 1-Gln 290
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, 15% glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 33.8 kDa
Observed MW: 37 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only