Recombinant Human Carbonic Anhydrase II/CA2 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031582-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase II; CA2
Target Symptom: CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282
Research Area: Cell biology; Cardiovascular
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: C-His
Accession: NP_000058.1
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Activity: Measured by its esterase activity. The specific activity is > 150 pmoles/min/μg.
Sequence: Met 1-Lys 260
Purity: > 96 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris 0.5M NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 30 kDa
Observed MW: 30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only