Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031569-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CPA2
Target Symptom: Carboxypeptidase A2; CPA2
Research Area: Cell biology
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
Accession: NP_001860.2
Background: Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5, 5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13, 260 M-1 cm-The specific activity is > 4, 000 pmoles/min/μg.
Sequence: Met 1-Tyr 417
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 46 kDa
Observed MW: 46 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only