Recombinant Human Cathepsin D/CTSD Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030822-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cathepsin D; CTSD
Target Symptom: Cathepsin D; CTSD; CLN10; CPSD; HEL-S-130P
Research Area: Signal Transduction; Cell biology; Neuroscience; Cancer;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: P07339
Accession: P07339
Background: Cathepsin D (CTSD); a well known lysosomal aspartyl protease and belongs to the peptidase C1 family; which is a normal and major component of lysosomes; and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments; other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins; the generation of bioactive proteins; antigen processing; etc. Among different role in cell physiology; a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition; CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin; a potent endogenous inhibitor of angiogenesis; suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.
Activity: Measured by its ability to bind biotinylated human CTSS-His in a functional ELISA.
Sequence: Met 1-Leu 412
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 25mM MES, 150mM NaCl, pH 6.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44 kDa
Observed MW: 40-110 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only