Recombinant Human Cathepsin S/CTSS Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033736-10, PKSH033736-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cathepsin S; CTSS
Target Symptom: Cathepsin S; CTSS; CTSS; MGC3886
Research Area: Tags & Cell Markers; Cell biology; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: P25774
Accession: P25774
Background: Cathepsin S is a lysosomal enzyme that belongs to the papain family of cysteine proteases. This protein is expressed by antigen presenting cells including macrophages, B-lymphocytes, dendritic cells and microglia. Moreover, cathepsin S is expressed in some epithelial cells. Compared with the abundant cathepsins B, LandH, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicated that cathepsin S generates A beta from amyloidogenic fragments of beta APP in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of Alzheimer’s disease(AD) and Down Syndrome (DS).
Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 The specific activity is 9063. 642 pmol/min/µg.
Sequence: Gln17-Ile331(pro)&Ser109-Ile331&Gln17-Ser109(Propeptide)
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 10% Glycerol, pH 5.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 36.9&25&11.2 kDa
Observed MW: 37-40&26-28&14-16 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only