Recombinant Human CD226/DNAM-1 Protein (Fc Tag)(Active)
Size: 50μg
Catalogue Number: PKSH033729-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD226; DNAM-1
Target Symptom: CD226 antigen; DNAX accessory molecule 1; DNAM-1; CD226; DNAM1
Research Area: immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: Q15762
Accession: Q15762
Background: Human DNAX accessory molecule 1 (DNAM-1/CD226) is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM-1 contains an extracellular domain (ECD) with two Ig-like C2-set domains and a cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155 and CD112. Ligation of DNAM-1 promotes the activation of NK cells; CD8+ T cells; and mast cells; dendritic cell maturation; megakaryocyte and activated platelet adhesion to vascular endothelial cells; and monocyte extravasation; it inhibits the forrmation of osteoclasts. Plateletendothelium; interactions mediated by DNAM-1; enable the metastasis of tumor cells to the lung.
Activity: Immobilized Human DNAM-1-Fc at 2μg/ml(100 μl/well) can bind Human Nectin-2-6His(Cat: PKSH032913). The ED50 of Human DNAM-1-Fc is 0.79 ug/mL
Sequence: Glu19-Asn247
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 52.8 kDa
Observed MW: 72-90 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only