Recombinant Human CD3D & CD3E Heterodimer Protein (Active)
Size: 50μg
Catalogue Number: PKSH030479-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD3D & CD3E Heterodimer
Target Symptom: T-Cell Surface Glycoprotein CD3 Delta Chain; T-Cell Receptor T3 Delta Chain; CD3d; CD3D; T3D
Research Area: immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Flag & hFc
UNIProt ID: P04234&P07766
Accession: P04234-1&P07766-1
Background: T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.
Sequence: Met 1-Ala105&Met 1-Asp126
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 77.5 (37.5+40） kDa
Observed MW: 45-50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only