Recombinant Human CD40L/TNFSF5 Protein (Human Cells)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032214-10, PKSH032214-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD40L; TNFSF5
Target Symptom: CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
Research Area: Cardiovascular; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: None
UNIProt ID: P29965
Accession: P29965
Background: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members; CD40LG exists as a trimer in membrane bound and soluble form; both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2.
Activity: Immobilied Anti-Human CD40L mAb at 5 ug/ml (100 ul/well) can bind Human CD40L. The EC50 of Human CD40L is not higher than 10 ng/ml.
Sequence: Met 113-Leu261
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.2 kDa
Observed MW: 16-18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only