Recombinant Human CD47/IAP Protein (Fc Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032703-10, PKSH032703-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD47; IAP
Target Symptom: Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6
Research Area: Cancer; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
UNIProt ID: Q08722
Accession: Q08722
Background: CD47(Integrin-Associated Protein; IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.
Activity: Immobilized Human SIRPA-His(Cat: PKSH033525) at 0.8μg/ml(100 μl/well) can bind Human CD47-Fc. The ED50 of Human CD47-Fc is 0.135 ug/ml.
Sequence: Gln19-Pro139
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 41.9 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only