Recombinant Human CD62L/L-Selectin protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100294-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD62L; L-Selectin
Target Synonym: L-selectin; Sell; CD62 antigen-like family member L; Leukocyte adhesion molecule 1 (LAM-1); Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; Lymphocyte antigen 22 (Ly-22); Lymphocyte surface MEL-14 antigen; CD62L
UNIProt ID: P30836
Research Areas: Stem Cells; Cardiovascular; Immunology; signal Transduction
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P30836
Background: L-Selectin (also known as Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L-Selectin share 76% amino acid (aa) sequence homology. L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
Sequence: Trp 39-Asn 332
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 32.23 kDa
Observed MW: 39 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only