Recombinant Human Cerberus/CER1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032240-10, PKSH032240-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cerberus; CER1
Target Synonym: Cerberus; Cerberus-Related Protein; DAN Domain Family Member 4; CER1; DAND4
UNIProt ID: O95813
Research Areas: Developmental Biology; immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: O95813
Background: Cerberus 1 is a secreted glycoprotein that forms disulfide-linked homodimers. It is a cytokine member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus, DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands. At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh. Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. The inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation. In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction.
Sequence: Thr18-Ala267
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM HAc.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 29.2 kDa
Observed MW: 35-42 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only