Recombinant Human CGREF1/CGR11 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032243-10, PKSH032243-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CGREF1; CGR11
Target Synonym: Cell Growth Regulator with EF Hand Domain Protein 1; Cell Growth Regulatory Gene 11 Protein; Hydrophobestin; CGREF1; CGR11
UNIProt ID: Q99674
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q99674
Background: Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
Sequence: Ala20-Ile301
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM GaCl2, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 30.9 kDa
Observed MW: 17-36 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only