Recombinant Human CNDP1 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031891-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CNDP1
Target Symptom: Beta-Ala-His Dipeptidase; CNDP Dipeptidase 1; Carnosine Dipeptidase 1; Glutamate Carboxypeptidase-Like Protein 2; Serum Carnosinase; CNDP1; CN1; CPGL2; HsT2308
Research Area: Signal Transduction; Cancer;
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
Accession: NP_116038.4
Background: CNDP1; also known as carnosine dipeptidase 1; glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1); is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region; which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy; for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans; CNDP1 is secreted from the liver into the serum. In other mammals; including rodents; CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β -alanyl-L-histidine); and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans; but not African-Americans. It was identified and confirmed as a risk factor; were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition; CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.
Activity: Measured by its ability to cleave carnosine (βAlaLHis) in a twostep assay. The specific activity is > 250 pmoles/min/μg.
Sequence: Ser27-His507
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 55.3 kDa
Observed MW: 60-65 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only