Recombinant Human Cyclophilin C/PPIC Protein (His&Trx Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032316-10, PKSH032316-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cyclophilin C; PPIC
Target Synonym: Peptidyl-Prolyl Cis-Trans Isomerase C; PPIase C; Cyclophilin C; Rotamase C; PPIC; CYPC
UNIProt ID: P45877
Research Areas: Signal Transduction; immunology;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-Trx-His
Accession: P45877
Background: Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but prolines unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Proteins with prolyl isomerase activity include cyclophilin, FKBPs, and parvulin, although larger proteins can also contain prolyl isomerase domains.
Sequence: Lys31-Asp182
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.8 kDa
Observed MW: 34 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only