Recombinant Human Cytochrome C/CYCS Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032336-10, PKSH032336-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cytochrome C; CYCS
Target Synonym: Cytochrome C; CYCS; CYC
UNIProt ID: P99999
Research Areas: Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: P99999
Background: Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase). CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Sequence: Gly2-Glu105
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 200mM NaCl, 50% Glycerol, 0.05% Tween 80, pH7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 12.8 kDa
Observed MW: 16 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only