Recombinant Human Deoxycytidine Kinase/DCK Protein (His &T7 Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032348-10, PKSH032348-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Deoxycytidine Kinase; DCK
Target Synonym: Deoxycytidine Kinase; dCK; DCK
UNIProt ID: P27707
Research Areas: Signal Transduction; Cancer;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P27707
Background: Deoxycytidine Kinase (DCK) is a member of the DCK/DGK family. DCK exists as a homodimer and is localized to the nucleus. DCK is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). DCK has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. In addition, DCK is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Sequence: Met 1-Leu260
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 34.0 kDa
Observed MW: 30 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only